A platform incorporating trimeric antigens into self‑assembling nanoparticles reveals SARS‑CoV‑2‑spike nanoparticles to elicit substantially higher neutralizing responses than spike alone

Antigens displayed on self-assembling nanoparticles can stimulate strong immune responses and have been playing an increasingly prominent role in structure-based vaccines. However, the development of such immunogens is often complicated by inefficiencies in their production. To alleviate this issue, we developed a plug-and-play platform using the spontaneous isopeptide-bond formation of the SpyTag:SpyCatcher system to display trimeric antigens on self-assembling nanoparticles, including the 60-subunit Aquifex aeolicus lumazine synthase (LuS) and the 24-subunit Helicobacter pylori ferritin.

A platform incorporating trimeric antigens into self‑assembling nanoparticles reveals SARS‑CoV‑2‑spike nanoparticles to elicit substantially higher neutralizing responses than spike alone
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