Detection of SARS-CoV-2 with SHERLOCK One-Pot Testing

based diagnostic tests collectively provide a nascent platform for the detection of viral and bacterial pathogens. Methods such as SHERLOCK (specific high-sensitivity enzymatic reporter unlocking), which typically use a two-step process (target amplification followed by CRISPR-mediated nucleic acid detection),1,2 have been used to detect SARS-CoV-2.3 These approaches, however, are more complex than those used in point-of-care testing because they depend on an RNA extraction step and multiple liquid-handling steps that increase the risk of cross-contamination of samples.

Detection of SARS-CoV-2 with SHERLOCK One-Pot Testing
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